Composite

Part:BBa_K914004:Design

Designed by: Dylan Iverson   Group: iGEM12_Paris_Bettencourt   (2012-09-20)


Pbad/AraC RBS LacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1187
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Removed LVA tag from LacI. Intended for use on low copy plasmid.


Source

Modified from a part from Paris Bettencourt 2009 iGEM team.

References